| Ashland | The Neighborhood, 2516 Carter Avenue | – | – | – | – | 11-2 | – | |
| Barbourville | Knox County Health Department, 261 Hospital Dr. | – | – | – | 1-3 | – | – | |
| Bardstown | Nelson County Health Department, 325 S. Third Street | 8-12, 1-4 | 8-12, 1-4 | 8-12, 1-4 | 8-12, 1-4 | 8-12, 1-4 | – | |
| Berea | Madison County Health Department, 1001 Ace Drive | – | – | – | 1-4 | – | – | |
| Booneville | Owsley County Health Department, 501 Highway 28 | 8-4 | 8-4 | 8-4 | 8-4 | 8-4 | – | |
| Bowling Green | Barren River District Health Department, 1109 State Street | – | – | – | 12-4 | – | – | |
| Columbia | Adair County Health Department, 801 Westlake Drive | 8-4:30 | 8-4:30 | 8-4:30 | 8-4:30 | 8-4:30 | – | |
| Cynthiana | Harrison County Health Center, 364 Oddville Ave. | – | – | – | 12-4:30 | – | – | |
| Danville | Boyle County Health Center (basement), 448 S 3rd Street | – | – | – | – | 1-3 | – | |
| Falmouth | Pendleton County Health Center, 329 KY-330 | 1-3 | – | – | – | – | – | |
| Frankfort | Franklin County Health Department, 100 Glenns Creek Road | 12-3 | 12-3 | 12-3 | 12-3 | 12-3 | – | |
| Grayson | Carter County Health Department, 300 West Main Street | 8-3 | 8-3 | 8-3 | 8-3 | 8-3 | – | |
| Greenup | Greenup County Health Department, 806 Seaton Ave # 1 | – | – | 1-4 | – | – | – | |
| Harrodsburg | Mercer County Health Department, 900 N. College Street | 8-4:30 | 8-6 | 8-4:30 | 8-6 | 8-12:30 | – | |
| Jamestown | Russell County Health Department, 211 Fruit of the Loom Drive | 8-4:30 | 8-4:30 | 8-4:30 | 8-4:30 | 8-4:30 | – | |
| Lancaster | Garrard County Health Center, 89 Farra Drive | 8-4:30 | 8-6 | 8-4:30 | 8-4:30 | 8-12 | – | |
| Lexington | Lexington-Fayette County Health Department, Dr. Rice C. Leach Community Room, 650 Newtown Pike | – | – | – | – | 11-4:30 | – | |
| Louisville | Hope Garden, 513 S. 26th St. | | | | | 9-12 | | |
| Louisville | Louisville Metro Public Health & Wellness, 400 E. Gray St. | 11-4 | | 1-6 | | 11-4 | | |
| Louisville | Lake Dreamland Fire Station, 4603 Cane Run Rd | – | – | – | 11-2 | – | – | |
| Louisville | 1455 Bicknell Ave. (corner of Bicknell and Taylor) | – | – | 11-2 | – | – | – | |
| Louisville | Portland Family Health Center parking lot, 2215 Portland Avenue | – | – | – | – | 1-4 | – | |
| Louisville | Redeemer Lutheran Church, 3640 River Park Drive | – | 11-2 | – | – | – | – | |
| Louisville | Beulah Presbyterian Church 6704 Bardstown Rd | | 3:30-6:30 (summer hours) | | | | | |
| Louisville | Social Practice Lab, 1229 S. Shelby St. | | 11-4 | | 11-4 | | 11-2 | |
| Louisville | St. John, 700 E. Muhammad Ali Blvd | | 9-12 | | | | | |
| Manchester | Clay County Health Department, 330 Shamrock Road | – | – | – | – | 12:30-3:30 | – | |
| Maysville | Mason County Health Department, 130 East Second Street | – | 2-4 | – | – | – | – | |
| McKee | Jackson County Health Department, 456 Main Street South | – | – | – | – | 12-3:30 | – | |
| Morehead | Rowan County Health Department, 730 West Main Street | – | 2-4 | – | – | – | – | |
| Mt. Olivet | Robertson County Health Department, 45 McDowell Street | – | – | – | 2-4:30 | – | – | |
| Nicholasville | Jessamine County Health Department, 210 East Walnut St. | 3-6 | – | – | – | – | – | |
| Pikeville | Pike County Health Department, 119 River Drive | 4:30-5 | – | – | – | – | – | |
| Prestonsburg | Floyd County Health Department, 283 Goble Street | – | – | 3-6 | – | – | – | |
| Richmond | Madison County Health Department, Gibson Lane | – | – | 1-4 | – | – | – | |
| Sandy Hook | Elliott County Health Center, 109 David Blair Blvd. | – | – | 2-4 | – | – | – | |
| Stanford | Lincoln County Health Department, 44 Health Way | 8-4 | 8-5 | 8-4 | 8-4 | – | – | |
| Stanton | Powell County Health Department, 376 North Main Street | – | – | – | 5:30-7 | – | – | |
| Williamsburg | Whitley County Health Department, 368 Penny Lane | – | – | – | – | 2-4 | – | |
| Williamstown | Grant County Health Center, 234 Barnes Road | – | – | 1-4 | – | – | – | |
| Winchester | Clark County Health Department, 400 Professional Avenue | – | – | – | – | 12-4 | – | |
